| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4766546 | Electrochemistry Communications | 2016 | 4 Pages |
•Aptamer-based neuropeptide Y detection at 10 pM levels without labeling and wash steps•High sensitivity detection using aptamer functionalized graphene-gold nanocomposites•Rapid detection from dilute samples by coupling aptamers to enriched neuropeptide Y•Patterned aptamers on a disposable nanoslit chip fabricated by imprint lithography•Verified for absence of interferences from other tyrosine containing peptides
We present an electrokinetically enhanced aptamer sensing platform on a disposable plastic chip for label-free detection of neuropeptide Y (NPY), which is a key neurological biomarker. The sensor consists of aptamer-functionalized graphene-gold nanocomposites (Gr-AuNs) patterned inside a nanoslit that is embossed on cyclic olefin copolymer via nanoimprint lithography. Analyte molecules are dielectrophoretically focused through the nanoslit onto aptamer-immobilized Gr-AuNs for rapid and selective electrochemical detection of NPY at picomolar levels.
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