Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4811 | Biochemical Engineering Journal | 2007 | 4 Pages |
Abstract
Dextransucrase forms a complex with dextran during an enzymatic reaction with sucrose. Using its enzymatic character, dextransucrase was immobilized on the pore surface of an inorganic membrane at 1.5 U/g-membrane, and subsequent permeation of a sucrose solution (25 g/L) was used to produce dextran on its surface. The pressure loss in the sucrose solution permeating the enzyme-immobilized membrane increased exponentially, indicating that the produced dextran filled the pore. A solution containing latex particles was permeated through the dextran-containing membrane for determining pore filling with dextran, demonstrating that the rejection percentage of latex bead increased with increasing the amount of dextran produced.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Hidetaka Kawakita, Hirokazu Seto, Keisuke Ohto, Katsutoshi Inoue, Hiroyuki Harada,