Article ID Journal Published Year Pages File Type
4825 Biochemical Engineering Journal 2006 8 Pages PDF
Abstract

Screening of biosurfactant-producing ability of four Lactobacillus strains was performed, being shown that for all the tested strains biosurfactant production occurred mainly in the first 4 h. The Lactobacillus strains showed zones of clearing in the blood agar with a diameter <1 cm. The minimum surface tension value of the fermentation broth achieved was 39.5 mN/m for Lactobacillus pentosus CECT-4023 that represents a reduction in the surface tension of 10.5 mN/m comparing with the control.Time courses of glucose, biomass and biosurfactant were modeled according to reported models. Using MRS broth (Man, Rogosa & Sharpe medium for lactobacilli strains) as culture medium, the values estimated by the modeling of biosurfactant were Pmax = 1.6 g of biosurfactant/L and rp/X = 0.091 g/(L h), for Lactobacillus casei CECT-5275, Pmax = 1.7 g/L and rp/X = 0.217 g/(L h) for Lactobacillus rhamnosus CECT-288, Pmax = 1.7 g/L and rp/X = 0.069 g/(L h) for L. pentosus CECT-4023 and Pmax = 1.8 g/L and rp/X = 0.090 g/(L h) for Lactobacillus coryniformis subsp. torquens CECT-25600. Pmax is the maximum concentration of biosurfactant (g/L), and Pr is the ratio between the initial volumetric rate of product formation (rp) and the initial product concentration P0 (g/L). Using whey as production medium, the values estimated by the modeling of biosurfactant for L. pentosus CECT-4023 were Pmax = 1.4 g of biosurfactant/L and rp/X = 0.093 g/(L h).In conclusion, the results obtained for L. pentosus CECT-4023 showed that this is a strong biosurfactant producer strain and that cheese whey can be used as an alternative medium for biosurfactant production.

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Physical Sciences and Engineering Chemical Engineering Bioengineering
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