Enzymatic polymerization of o-phenylendiamine with cytochrome c activated by a calixarene derivative in organic media

Cytochrome c can be dissolved in organic media through complexation with a macrocyclic compound calix[6]arene carboxylic acid derivative. Enzymatic oxidation of o-phenylenediamine with the cytochrome c complex as a catalyst was demonstrated in organic media. The cytochrome c complex displays peroxidase activity in the presence of hydrogen peroxide. Enzymatic polymerization of o-phenylenediamine was examined under optimal conditions. A polymer product was effectively obtained in hexane or chloroform; however, a monomeric compound was also in the product. In this paper, a novel solubilization method utilizing enzymes and their uses as catalysts in organic media are described.