Article ID Journal Published Year Pages File Type
4910990 Procedia Chemistry 2016 4 Pages PDF
Abstract

An amperometric nitrate biosensor was successfully constructed by the electroimmobilization of the enzyme nitrate reductase from Aspergillus niger with and without β-NADH, a co-factor, into conducting polypyrrole films on screen printed carbon electrode (SPCE). The best performance was registered by nitrate biosensor developed without the β-NADH electroimmobolize with nitrate reducatse on the SPCE. The formation of the optimum NaR-PPy composite on the SPCE was achieved with an applied potential of 0.9 Vs-1 for 900s. The optimum amperometric response for nitrate was obtained in 0.1M potassium phosphate buffer, pH 7.5 with an applied potential of -0.1 Vs-1 and 2mM β-NADH. Sensitivity of the nitrate biosensor was found to be in a linear range of 0.01-0.25mM with a regression coefficient of 0.97 and a relative standard deviation (RSD) of 6.6% (n=5).

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Physical Sciences and Engineering Chemical Engineering Chemical Engineering (General)
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