| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4962355 | Procedia Technology | 2017 | 4 Pages |
Abstract
The first amperometric immunosensor for the quantification of TGF-β1 is described in this work. The immunosensor design involves disposable devices using carboxylic acid-functionalized magnetic microparticles supported onto screen-printed carbon electrodes and covalent immobilization of the specific antibody for TGF-β1 using Mix&GoTM polymer. A sandwich-type immunoassay was performed using peroxidase-labeled streptavidin (Poly-HRP-Strept) polymer for signal amplification. The calibration plot allowed a range of linearity extending between 15 and 3000 pg/mL TGF-β1 (LOD 10 pg/mL). The usefulness of the immunosensor was evaluated by analyzing spiked urine at different pg/mL concentration levels.
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Authors
E. Sánchez-Tirado, A. González-Cortés, P. Yánez-Sedeño, J.M. Pingarrón,