Rapid naked eye detection of alkaline phosphatase using α-MoO3-x nano-flakes

•We propose to develop novel naked eye sensor for alkaline phosphatase.•α-MoO3-x is used as Rapid detection tool for sensing alkaline phosphatase in presence of Zinc in ppm.•The method has also been tested for detection of alkaline phosphatase in biological fluids to affirm the sensitivity in real samples.

We present a novel platform enabled for rapid naked eye detection of alkaline phosphatase using two-dimensional α-MoO3-x nano-flakes as reporter molecule as first of its kind report. The detection system involved α-MoO3-x nano-flakes and zinc oxide as the primary constituents followed by the introduction of alkaline phosphatase. The rapid transformation of α-MoO3-x (blue) to α-MoO3 (colorless) in presence of alkaline phosphatase with zinc as a cofactor is an indication for the presence of the enzyme. The sensitivity of the current biosensor was found to reach 0.1 μM of enzyme concentration. The stability and selectivity of the novel biosensor has been tested at different pH and with a wide range of analytes in the serum.