Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5009051 | Sensors and Actuators B: Chemical | 2017 | 10 Pages |
â¢A new nanoaptasensor was introduced for detection of troponin I.â¢This aptasensor could detect troponin I in a wide linear range a low limit of detection.â¢The aptasensor was applied in the analysis of 89 real samples.
Recently, convergence of nanotechnology, medicine, biology and chemistry has provided novel solutions for efficient diagnosis systems for disease markers. A detection method of troponin I (TnI), as the gold standard of acute myocardial infarction biomarker, was investigated and a novel aptasensor was presented for this biomarker. An array of gold nanodumbbells was firstly synthesized using putrescine as the shape directing agent at a surface. It was then applied as a transducer for the immobilization of a 76-mer TnI aptamer to fabricate a simple electrochemical TnI aptasensor. Using the aptasensor, TnI was detected in a linear range of 0.05-500 ng mLâ1 with a limit of detection of 8.0 pg mLâ1. The aptasensor showed a diagnostic sensitivity of 100% and a diagnostic specificity of 85% when challenged with blood serum samples of 89 individuals.