Biomolecular kinetics analysis using long-range surface plasmon waveguides

A novel, cost effective, label free and real time biosensor based on straight gold waveguides supporting long range surface plasmon polaritons (LRSPPs) is used to measure the kinetics constants of protein-protein interactions. Bovine serum albumin (BSA) and anti-bovine albumin antibody (anti-BSA) produced in rabbit are used as the bio-pair for the purposes of demonstrating and investigating the extraction of binding kinetics. BSA protein is immobilized on the surface using thiol coupling. The output power of the system is recorded continuously over time, and then converted into surface mass concentration. The kinetics constants are extracted from binding curves using the integrated rate equation. Linear least squares and non-linear least squares analysis are employed to obtain the constants and the results are compared.