Smart naturally plastic antibody based on poly(α-cyclodextrin) polymer for β-amyloid-42 soluble oligomer detection

The ability of the BIOPLAST film to rebind Aβ-42 was followed by electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV), and compared to a control film without plastic antibody (NCd/PANI/Au-SPE). The MCd/PANI/Au-SPE devices displayed linear responses and limit of detection to Aβ-42 in EIS and SWV assays, down to 0.25 ng/mL and 0.20 ng/mL respectively. The dissociation constant between the BIOPLAST and Aβ-42 were 0.2 ± 0.5 ng/mL (1.8 ± 0.5 for the control material). MCd/PANI/Au-SPE materials assembled on screen-printed electrodes showed great promising features, regarding its possible application in point-of-care.