Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5009713 | Sensors and Actuators B: Chemical | 2017 | 23 Pages |
Abstract
Patients with pancreatic and colorectal cancers have elevated levels of activated leukocyte cell adhesion molecule (ALCAM) in their blood compared to healthy individuals. To this end, sensitive detection of soluble fraction of ALCAM (sALCAM), also called CD166, was performed using enzyme-linked antibody aptamer (ELAA) assay. For the assay, aptamer specific for sALCAM was used as a capturing probe and polyclonal antibody modified with an enzyme was used as a detecting probe for colorimetric visualization. The ELAA assay was able to detect sALCAM as low as 0.31Â ng/mL. In addition, the assay enabled the differentiation of sALCAM from other proteins in buffer as well as in human serum. The ELAA assay provides cost-effective, highly sensitive, and reproducible detection of sALCAM.
Keywords
CD166interleukin-5 receptorInterleukin-13 receptorSGIIL-17Rinterleukin-17 receptorIL-5RIL-13RSELEXBSASybr Green Ibovine serum albuminAptamerELISAEnzyme-linked immunosorbent assaySurface plasmon resonanceSPRsystematic evolution of ligands by exponential enrichmentALCAMactivated leukocyte cell adhesion molecule
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jin Her, Hunho Jo, Changill Ban,