Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5010064 | Sensors and Actuators B: Chemical | 2017 | 7 Pages |
Abstract
We report on a study of enzymatic glucose and lactate sensors for measurements of wide concentration ranges from 1Â mM up to 600Â mM and 900Â mM, respectively, in biotechnological processes. Diffusion-limiting polyurethane membranes were used to extend the linear measurement range and the influence of two fabrication parameters on membrane properties and sensor performance evaluated. The polymer concentration was varied between 4% and 10% and the ratio of the solvents tetrahydrofuran and dimethylformamide between 9:1 and 1:9. Surface morphology and permeability of the membranes and the linear measurement range of membrane-covered enzyme sensors were determined. A distinct relationship between the different results was found. The sensors retained their functionality after being sterilized using gamma and electron beam irradiation. The long-term performance of the sensors was evaluated and good performance in the glucose monitoring of a culture of Saccharomyces cerevisiae over 13Â days was shown.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Stefan Mross, Tom Zimmermann, Sebastian Zenzes, Michael Kraft, Holger Vogt,