Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5130660 | Analytica Chimica Acta | 2017 | 8 Pages |
â¢A single-labeled and dual-functional probe was cleverly designed.â¢Simple and cost-efficient fluorescence sensor was applied to quantify miRNA.â¢Simultaneous detection of miRNA-122 and miRNA-26a was successfully achieved.
The simultaneous detection of two important microRNAs (miRNAs) can potentially evaluate pathological states. The simultaneous detection of miRNA-26a and miRNA-122 is proposed in this study using a cleverly designed single-labeled, dual-functional fluorescent probe with a 2-aminopurine as a fluorophore, which is a G-quadruplex single-stranded DNA. The probe can partly complement cDNA-1 and cDNA-2. cDNA-1 and cDNA-2 are complementary strands of miRNA-26a and miRNA-122, respectively. When the target miRNAs are added simultaneously, these cDNA (cDNA-1 and cDNA-2) can be competed off from the cDNAâprobe duplex to form a cDNAâRNA heteroduplex. The probe is released and the fluorescent signal is increased. The detection limits of miRNA-26a and miRNA-122 are both 2.5Â nM, and their wide linear which ranges from 2.5 to 500Â nM are achieved using the assay. This method has potential practical applications.
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