Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5131010 | Analytica Chimica Acta | 2017 | 7 Pages |
â¢A sandwich-type immunoassay is developed to detect β-amyloid.â¢The assay utilizes foldamer helices as recognition elements.â¢The assay is selective to β-amyloid oligomers against monomeric forms.â¢Î²-amyloid oligomers can be detected at pM levels.
Mimicking the molecular recognition functionality of antibodies is a great challenge. Foldamers are attractive candidates because of their relatively small size and designable interaction surface. This paper describes a sandwich type enzyme-linked immunoassay with a tetravalent β-peptide foldamer helix array as capture element and enzyme labeled tracer antibodies. The assay was found to be selective to β-amyloid oligomeric species with surface features transiently present in ongoing aggregation. In optimized conditions, with special emphasis on the foldamer immobilization, a detection limit of 5 pM was achieved with a linear range of 10-500 pM. These results suggest that protein mimetic foldamers can be useful tools in biosensors and affinity assays.
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