Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5131211 | Analytica Chimica Acta | 2017 | 8 Pages |
â¢Cross-validation of analytes in complex samples was done with different CE separation mechanisms.â¢A simple strategy is used to confirm peak identification and extend capacity of CE separation.â¢The method uses small amount of sample, simple instrument and single fluorescent labeling.â¢Selection of mechanisms is based on orthogonalities of GU values of glycan standards.â¢Micellar electrokinetic chromatography was suitable for analysis of small or highly sialylated glycans.
The deep involvement of glycans or carbohydrate moieties in biological processes makes glycan patterns an important direction for the clinical and medicine researches. A multiplexing CE mapping method for glycan analysis was developed in this study. By applying different CE separation mechanisms, the potential of combined parallel applications of capillary zone electrophoresis (CZE), micellar electrokinetic chromatography (MEKC) and capillary gel electrophoresis (CGE) for rapid and accurate identification of glycan was investigated. The combination of CZE and MEKC demonstrated enhancing chromatography separation capacity without the compromises of sample pre-treatment and glycan concentration. The separation mechanisms for multiplexing platform were selected based on the orthogonalities of the separation of glycan standards. MEKC method exhibited promising ability for the analysis of small GU value glycans and thus complementing the unavailability of CZE. The method established required only small amount of samples, simple instrument and single fluorescent labelling for sensitive detection. This integrated method can be used to search important glycan patterns appearing in biopharmaceutical products and other glycoproteins with clinical importance.
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