Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5131287 | Analytica Chimica Acta | 2016 | 7 Pages |
â¢We developed a simple system for Fe3+ and Cu2+ sensing with low cost and easy operations.â¢Fe3+ and Cu2+ are simultaneously detected via colorimetric readout and fluorescent absorbance signals.â¢The sensor realized the simultaneously detection of Fe3+ and Cu2+ successively with highly sensitivity and selectivity.
Here, we report an ultra-sensitive and colorimetric sensor for the detection of Fe3+ or Cu2+ successively using glutathione-functionalized Au nanoclusters (GSH-AuNCs). For GSH-AuNCs can catalytically oxidize peroxidase substrates, such as 3, 3â², 5, 5â²-tetramethylbenzidine (TMB), colored products are formed in the presence of H2O2. While upon the addition of Fe3+ or Cu2+ into the GSH-AuNCs-TMB-H2O2 system, diverse color and absorbance of the system was obtained due to the self oxidation of Fe3+ and the inhibition of peroxidase-like activity of GSH-AuNCs. With the introduction of ethylene diamine tetraacetic acid (EDTA) or ammonium fluoride (NH4F) to GSH-AuNCs-TMB-H2O2+Cu2+ system or GSH-AuNCs-TMB-H2O2+Fe3+ system respectively, a restoration of color and absorbance of system was realized. On the basis of above phenomenon, a colorimetric and quantitative approach for detecting Fe3+ and Cu2+ was developed with detection limit of 1.25Â ÃÂ 10â9Â M and 1.25Â ÃÂ 10â10Â M respectively. Moreover, the concentration of Fe3+ and Cu2+ in human serums was also accurate quantified by this method. So this design strategy realized the simple and simultaneous detection of Fe3+ and Cu2+, suggesting significant potential in clinical diagnosis.
Graphical abstractWe designed a novel strategy for the colorimetric and sensitive detection of Fe3+ and Cu2+ based on the peroxidase-like activity of GSH-AuNCs.Download full-size image