Tutorial on estimating the limit of detection using LC-MS analysis, part II: Practical aspects

•Different LOD determination approaches are compared from practical LC-MS viewpoint.•Specific aspects of LC-MS/MS are addressed.•Recommendations and a flow-chart of LOD determination in LC-MS are given.

In part II of this tutorial, the investigated approaches of estimating the limit of detection (LOD) are applied to experimental data from LC-MS measurements. Important practical aspects specific to LC-MS and related to LOD are reviewed. The results of different tests of estimating linearity and scedasticity are compared. LOD estimates obtained with different approaches (for both simple characterization of the analysis method and accurate interpretation of the results) are applied to the data and the obtained values are compared. As a conclusion, a decision tree is proposed for estimating LOD for analytical methods using the LC-MS technique.

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