| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5144952 | Bioelectrochemistry | 2018 | 8 Pages |
Abstract
Cytosolic, synthetic chemical calcium indicators are typically used to visualise the rapid increase in intracellular calcium ion concentration that follows nanosecond pulsed electric field (nsPEF) application. This study looks at the application of genetically encoded calcium indicators (GECIs) to investigate the spatiotemporal nature of nsPEF-induced calcium signals using fluorescent live cell imaging. Calcium responses to 44Â kV/cm, 10Â ns pulses were observed in U87-MG cells expressing either a plasma membrane targeted GECI (GCaMP5-G), or one cytosolically expressed (GCaMP6-S), and compared to the response of cells loaded with cytosolic or plasma membrane targeted chemical calcium indicators. Application of 100 pulses, to cells containing plasma membrane targeted indicators, revealed a wave of calcium across the cell initiating at the cathode side. A similar spatial wave was not observed with cytosolic indicators with mobile calcium buffering properties. The speed of the wave was related to pulse application frequency and it was not propagated by calcium induced calcium release.
Related Topics
Physical Sciences and Engineering
Chemistry
Electrochemistry
Authors
Lynn Carr, Sylvia M. Bardet, Delia Arnaud-Cormos, Philippe Leveque, Rodney P. O'Connor,