Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5144984 | Bioelectrochemistry | 2017 | 40 Pages |
Abstract
Multi-wall carbon nanotubes (MWCNTs) were modified to design a new DNA biosensor. Functionalized MWCNTs were equipped with gold nanoparticles (GNPs) (~ 15 nm) (GNP-MWCNTCOOH) to construct DNA biosensors based on carbon-paste screen-printed (SPE) electrodes. GNP attachment onto functionalized MWCNTs was carried out by microwave irradiation and was confirmed by spectroscopic studies and surface analysis. DNA biosensors based on differential pulse voltammetry (DPV) were constructed by immobilizing thiolated single-stranded DNA probes onto GNP-MWCNTCOOH. Ruthenium (III) chloride hexaammoniate [Ru(NH3)6,2Clâ] (RuHex) was used as hybridization redox indicator. RuHex and MWCNT interaction was low in compared to other organic redox hybridization indicators. The linear response range for DNA determination was 1 Ã 10â 21 to 1 Ã 10â 9 M with a lower detection limit of 1.55 Ã 10â 21 M. Thus, the attachment of GNPs onto functionalized MWCNTs yielded sensitive DNA biosensor with low detection limit and stability more than 30 days. Constructed electrode was used to determine gender of arowana fish.
Related Topics
Physical Sciences and Engineering
Chemistry
Electrochemistry
Authors
Kasra Saeedfar, Lee Yook Heng, Chew Poh Chiang,