Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5146096 | International Journal of Hydrogen Energy | 2017 | 11 Pages |
Abstract
Biohydrogen production from cellulose by a bacterial co-culture is a potentially promising approach for producing bioenergy from a low cost substrate. The use of a cellulolytic bacterium, Cellulomonas fimi, permits cellulose conversion and the in situ production of substrate for growth and hydrogen production by the photosynthetic bacterium Rhodopseudomonas palustris. Response surface methodology (RSM) with a Box-Behnken design (BBD) was used to examine variations in the key parameters: substrate (cellulose) concentration, yeast extract concentration and the microorganism ratio (Rps. palustris/C. fimi). For the co-culture of R. palustris and C. fimi the highest hydrogen production (44Â mmol H2/L) was achieved at the highest substrate concentration (5Â g/L); however, the highest hydrogen yield (3.84Â mol H2/mol glucose equivalent) was observed at the lowest cellulose concentration and highest microorganism ratio. High COD removal efficiencies, over 70%, were achieved over a wide range of conditions and were positively affected by the concentration of yeast extract.
Related Topics
Physical Sciences and Engineering
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Authors
Zeynep Yilmazer Hitit, Carolina Zampol Lazaro, Patrick C. Hallenbeck,