Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5147013 | International Journal of Hydrogen Energy | 2017 | 12 Pages |
Abstract
Hydrogen can be produced via dark and photo-fermentation using either single-stage or two-stage processes. The advantage of a two-stage system is that it is possible to separately optimize and control culture conditions for the dark and photo-fermentative bacteria. In the present study a mixture of starch and glucose was used as carbon source for the dark fermentation step. Response surface methodology (RSM) with a Box-Behnken design (BBD) was applied to the photo-fermentation stage for the optimization of key parameters: inoculum concentration (Rhodopseudomonas palustris), substrate concentration (dark fermentation effluent (DFE)) and pH. In this sequential two-stage system, the highest overall hydrogen yield (8.3 ± 0.1 mmol H2/gCOD), overall hydrogen production (1.62 mmol) and the photo fermentation yield (7.21 ± 0.2 mmol H2/gCOD) were achieved at inoculum of 9 mL (1.64 ± 0.7 Ã 108 cells), a DFE dilution of 2.5à and a pH of 7.5, which were center points of the design. 97% COD removal was achieved at the highest dilution of DFE (lowest concentration of carbon source) at pH 6.5.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Electrochemistry
Authors
Zeynep Yilmazer Hitit, Carolina Zampol Lazaro, Patrick C. Hallenbeck,