Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5148074 | International Journal of Hydrogen Energy | 2017 | 9 Pages |
Abstract
Hydrogen-producing bacterial strains were isolated from granular sludge from a UASB reactor that treats brewery wastewater. Most of the isolated strains were related to the Enterobacter genus through a phylogenetic analysis of the 16S rRNA sequences. The strains could use various carbon sources (sugars and glycerol) to produce hydrogen. The isolated strain, identified as Enterobacter sp. based on 16S rRNA gene sequencing, produced 6.8 mmol H2 Lâ1 culture medium when growing on glucose (2.0 g Lâ1) in anaerobic conditions at 30 °C. The main liquid metabolites were acetic acid (367 mg Lâ1), methanol (437 mg Lâ1) and ethanol (1101.26 mg Lâ1), after 9 h of fermentation. The maximum hydrogen yield of 0.8 mol H2/mol glucose was observed, indicating that Enterobacter sp. isolated from brewery wastewater was an efficient hydrogen-producing bacterium under mesophilic conditions.
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Authors
Sandra Imaculada Maintinguer, Carolina Zampol Lazaro, Renan Pachiega, Maria Bernadete Amâncio Varesche, Rodrigo Sequinel, José Eduardo de Oliveira,