Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5488778 | Current Applied Physics | 2017 | 34 Pages |
Abstract
In this paper, an electrosynthesized bilayer film of polypyrrole nanowire and poly(1,5diaminonaphthalene) on a disposable screen-printing carbon ink electrode is presented. The inner polypyrrole nanowire layer had a large surface-to-volume ratio and high conductivity in the neutral medium, whereas the outer layer of poly(1,5diaminonaphthalene) had functional amino groups on the polymer chains. The combination of polypyrrole nanowire and poly(1,5diaminonaphthalene) showed a promising material for electrochemical biosensing. Here we reported an electrochemical immunosensor based on this approach for the purpose of detecting breast cancer biomarkers. The bilayer could enhance the surface coverage of antibody anti-CA 15-3 and consequently improve the sensitivity and stability of the immunosensors. The magnetic beads were used as carriers of labeled antibody anti-CA15-3 and HRP (horseradish peroxidase) in order to achieve an amplification of the signal. Under optimized conditions, the linear range of the immunoassay was 0.05-20Â UÂ mLâ1 with a detection limit of 0.02Â UÂ mLâ1Â CA 15-3 antigen.
Related Topics
Physical Sciences and Engineering
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Condensed Matter Physics
Authors
Vân-Anh Nguyen, Huy L. Nguyen, Dzung T. Nguyen, Quan P. Do, Lam D. Tran,