Characterization of secondary structure and lipid binding behavior of N-terminal saposin like subdomain of human Wnt3a

Article ID	Journal	Published Year	Pages	File Type
5504311	Archives of Biochemistry and Biophysics	2017	9 Pages	PDF

Abstract

Structural model of Xenopus Wnt8 in complex with one the cysteine-rich domain of its co-receptor, frizzled 8 (grey space-fill). The saposin like domain within the overall Wnt structure is depicted in red.197

Keywords

Wnt3a dMPC CRD HRP GAPDH FZD N-terminal frizzled DTT FBS SDS DMEM DTAC SAPLIP 1,2-dimyristoyl-sn-glycero-3-phosphocholine 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide LRP5/6 Dulbecco's modified Eagle's medium MTT sld SDS-PAGE C-terminal cysteine-rich domain Dodecyltrimethylammonium chloride dithiothreitol circular dichroism Saposin sodium dodecyl sulfate fetal bovine serum Circular dichroism spectroscopy Liposomes Horseradish peroxidase Limited proteolysis Saposin-like protein CHAPS glyceraldehyde-3-phosphate dehydrogenase Guanidine hydrochloride

Related Topics

Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry

Characterization of secondary structure and lipid binding behavior of N-terminal saposin like subdomain of human Wnt3a

Authors

Aparna Krishnamoorthy, Andrzej Witkowski, Jesse J. Tran, Paul M.M. Weers, Robert O. Ryan,