Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5505282 | Biochemical and Biophysical Research Communications | 2017 | 7 Pages |
Abstract
The 4-phosphatase Inositol polyphosphate 4-phosphatase II (INPP4B) is a regulator of the PI3K signalling pathway and functions to suppress or promote activation of downstream kinases depending on cell type and context. Here we report the identification of a novel small transcript variant of INPP4B (INPP4B-S) that has a role in promoting proliferation of colon and breast cancer cells. INPP4B-S differed from full length INPP4B (INPP4B-FL) by the insertion of a small exon between exons 15 and 16 and the deletion of exons 20-24. Nevertheless, INPP4B-S retained all the functional domains of INPP4B-FL and was similarly located to the cytoplasm. Overexpression of INPP4B-S increased, whereas selective knockdown of INPP4B-S reduced the rate of proliferation in HCT116 and MCF-7Â cells. These results warrant further investigation of the role INPP4B-S in activation of downstream kinases and in regulation of cancer pathogenesis.
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Authors
Amanda Croft, Su Tang Guo, Simonne Sherwin, Margaret Farrelly, Xu Guang Yan, Xu Dong Zhang, Chen Chen Jiang,