Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5505290 | Biochemical and Biophysical Research Communications | 2017 | 5 Pages |
Abstract
Serotonin (5-hydroxytryptamine, 5-HT) plays an important role in milk volume homeostasis in the mammary gland during lactation; 5-HT in milk may also affect infant development. However, there are few reports on 5-HT concentrations in human breast milk. To address this issue, we developed a simple method based on high-performance liquid chromatography with fluorescence detection (HPLC-FD) for measuring 5-HT concentrations in human breast milk. Breast milk samples were provided by four healthy Japanese women. Calibration curves for 5-HT in each sample were prepared with the standard addition method between 5 and 1000Â ng/ml, and all had correlation coefficients >0.999. The recovery of 5-HT was 96.1%-101.0%, with a coefficient of variation of 3.39%-8.62%. The range of 5-HT concentrations estimated from the calibration curves was 11.1-51.1Â ng/ml. Thus, the HPLC-FD method described here can effectively extract 5-HT from human breast milk with high reproducibility.
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Authors
Takeshi Chiba, Tomoji Maeda, Tomohiko Tairabune, Takashi Tomita, Atsushi Sanbe, Rika Takeda, Akihiko Kikuchi, Kenzo Kudo,