Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5505330 | Biochemical and Biophysical Research Communications | 2017 | 6 Pages |
Abstract
Alkyl hydroperoxide reductase E (AhpE) is a member of the peroxidase family of enzymes that catalyse the reduction of peroxides, however its structural and functional roles are still unclear in details. In this study, we used the Thermococcus kodakarensis AhpE-like protein as a model to investigate structure-function relationships including the molecular properties of DNA binding activity. Multiple sequence alignment, structural comparison and biochemical analyses revealed that TkAhpE includes conserved peroxidase residues in the active site, and exhibits peroxidase activity with structure-dependent holdase chaperone function. Following electrophoretic mobility shift assays and electron microscopy analysis demonstrated distinctive binding features of TkAhpE to the DNA showing that their dimeric conformer can bind to the double-stranded DNA, but not to the single-stranded DNA, indicating its striking molecular features to double-stranded DNA-specific interactions. Based on our results, we provided that TkAhpE is a multifunctional peroxidase displaying structure-dependent molecular chaperone and DNA binding activities.
Keywords
SECmetal catalyzed oxidationThermococcus kodakarensisAhpEGRxPRXssDNADRETrxEtBrDTTdsDNACATMCOSingle-stranded DNAdouble-stranded DNAROSSORElectrophoretic mobility shift assayethidium bromideEDTAEthylenediaminetetraacetic acidthioredoxinresolving cysteineRoom temperaturedithiothreitolStructure-function relationshipSODEMSA یا electrophoretic mobility shift assay Superoxide reductaseSuperoxide dismutaseLuria-BertaniElectron microscopyPeroxidaseperoxidatic cysteineDNA binding proteinPeroxiredoxinCatalaseSize exclusion chromatographyGlutaredoxinReactive oxygen species
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Authors
Sangmin Lee, Hyeongseop Jeong, Ju Huck Lee, Jeong Min Chung, Rumi Kim, Hyung Joong Yun, Jonghan Won, Hyun Suk Jung,