Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5505332 | Biochemical and Biophysical Research Communications | 2017 | 30 Pages |
Abstract
Resveratrol is a flavonoid found in various plants including grapes, which has been reported to be active against various pathogenic bacteria. However, antibacterial effects and mechanisms via pro-oxidant property of resveratrol remain unknown and speculative. This research investigated antibacterial mechanism of resveratrol against a food-borne human pathogen Salmonella typhimurium, and confirmed the cell death associated oxidative damage. Resveratrol increased outer membrane permeability and membrane depolarization. It also was observed DNA injury responses such as DNA fragmentation, increasing DNA contents and cell division inhibition. Intracellular ROS accumulation, GSH depletion and significant increased malondialdehyde levels were confirmed, which indicated pro-oxidant activity of resveratrol and oxidative stress. Furthermore, the observed lethal damages were reduced by antioxidant N-acetylcysteine treatment supported the view that resveratrol-induced oxidative stress stimulated S. typhimurium cell death. In conclusion, this study expands understanding on role of pro-oxidant property and insight into previously unrecognized oxygen-dependent anti-Salmonella mechanism on resveratrol.
Keywords
DAPIDTNBMDANPNGSSGGSHNACMICN-phenyl-1-naphthylamine2′,7′-dichlorodihydrofluorescein diacetate4′,6-diamidino-2-phenylindole dihydrochloride5,5′-dithiobis(2-nitrobenzoic acid)DiBAC4(3)H2DCFDAN-acetylcysteineROSTerminal deoxynucleotidyl transferase dUTP nick end labelingOxidative stressTUNELMinimum inhibitory concentrationResveratrolSalmonella TyphimuriumOuter membraneAntibacterial activitymalondialdehydeProoxidantreduced glutathioneoxidized glutathioneReactive oxygen species
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Authors
Wonjong Lee, Dong Gun Lee,