Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5505508 | Biochemical and Biophysical Research Communications | 2017 | 6 Pages |
Abstract
We investigated the role of FAD2, which was predicted to encode a fatty acid desaturase of the n-alkane-assimilating yeast Yarrowia lipolytica. Northern blot analysis suggested that FAD2 transcription was upregulated at low temperature or in the presence of n-alkanes or oleic acid. The FAD2 deletion mutant grew as well as the wild-type strain on glucose, n-alkanes, or oleic acid at 30 °C, but grew at a slower rate at 12 °C, when compared to the wild-type strain. The growth of the FAD2 deletion mutant at 12 °C was restored by the addition of 18:2, but not 18:1, fatty acids. The amount of 18:2 fatty acid in the wild-type strain was increased by the incubation at 12 °C and in the presence of n-octadecane. In contrast, 18:2 fatty acid was not detected in the deletion mutant of FAD2, confirming that FAD2 encodes the Î12-fatty acid desaturase. These results suggest that Î12-fatty acid desaturase is involved in the growth of Y. lipolytica at low temperature.
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Authors
Satoshi Tezaki, Ryo Iwama, Satoshi Kobayashi, Yuh Shiwa, Hirofumi Yoshikawa, Akinori Ohta, Hiroyuki Horiuchi, Ryouichi Fukuda,