Identification and characterization of novel lncRNAs in Arabidopsis thaliana

•One hundred and fifty-six novel lncRNAs were identified and characterized in Arabidopsis thaliana.•Potential target genes of many lncRNAs were predicted and functional annotated.•Seventeen of the novel lncRNAs were predicted targets of 22 miRNAs.•Stage- and tissue-specific expression patterns of novel lncRNAs were examined by qRT-PCR.

Long noncoding RNAs (lncRNAs) are important regulators of various biological processes, but few studies have identified lncRNAs in plants; genome-wide discovery of novel lncRNAs is thus required. We used deep strand-specific sequencing (ssRNA-seq) to obtain approximately 62 million reads from all developmental stages of Arabidopsis thaliana and identified 156 novel lncRNAs that we classified according to their localization. These novel identified lncRNAs showed low expression levels and sequence conservation. Bioinformatic analysis predicted potential target genes or cis-regulated genes of 91 antisense and 32 intergenic lncRNAs. Functional annotation of these potential targets and sequence motif analysis indicated that the lncRNAs participate in various biological processes underlying Arabidopsis growth and development. Seventeen of the lncRNAs were predicted targets of 22 miRNAs, and a network of interactions between ncRNAs and mRNAs was constructed. In addition, nine lncRNAs functioned as miRNA precursors. Finally, qRT-PCR revealed that novel lncRNAs have stage- and tissue-specific expression patterns in A. thaliana. Our study provides insight into the potential functions and regulatory interactions of novel Arabidopsis lncRNAs, and enhances our understanding of plant lncRNAs, which will facilitate functional research.