Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5506945 | Biochemical and Biophysical Research Communications | 2016 | 5 Pages |
Abstract
Chloride ion (Clâ) is one of the most abundant anions in our body. Increasing evidence suggests that Clâ plays fundamental roles in various cellular functions. We have previously reported that electroneutral cation-chloride cotransporters, such as Na+-K+-2Clâ cotransporter 1 (NKCC1) and K+-Clâ cotransporter 1 (KCC1), are involved in neurite outgrowth during neuronal differentiation. In the present study, we studied if there is correlation between intracellular Clâ concentrations ([Clâ]i) and the length of growing neurites. We measured [Clâ]i in the cell body and growing neurite tips using halide-sensitive fluorescent dye N-(ethoxycarbonylmethyl)-6-methoxyquinolinium bromide (MQAE), revealing that [Clâ]i in the tip of growing neurite was higher than that in cell body in a single cell. Importantly, there was a significant positive correlation between the length of growing neurite and [Clâ]i in neurite tip. Bumtanide (BMT), an inhibitor of NKCC1, significantly inhibited neurite outgrowth and decreased [Clâ]i in neurite tip. The results obtained in the present study and our previous studies together strongly suggest that high [Clâ]i in neurite tip region is crucial for efficient neurite outgrowth.
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Authors
Ken-ichi Nakajima, Yoshinori Marunaka,