Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5506965 | Biochemistry and Biophysics Reports | 2017 | 8 Pages |
â¢IL-6-induced COX-2 expression was attenuated by kaempferol in macrophages.â¢The attenuation is attributed to simultaneous deactivation of STAT3 and NF-κB.â¢The nuclear translocation of both transcription factors are prevented by kaempferol treatment.â¢Kaempferol targets STAT3 and NF-κB and inhibits COX-2 expression to reduce carrageenan-induced mouse paw edema.
Cycloxygenase-2 (COX-2) is the inducible isoform of cycloxygenase enzyme family that catalyzes synthesis of inflammatory mediators, prostanoids and prostaglandins, and therefore, can be targeted by anti-inflammatory drugs. Here, we showed a plant polyphenol, kaempferol, attenuated IL-6-induced COX-2 expression in human monocytic THP-1 cells suggesting its beneficial role in chronic inflammation. Kaempferol deactivated and prevented nuclear localization of two major transcription factors STAT3 and NF-κB, mutually responsible for COX-2 induction in response to IL-6. Moreover, STAT3 and NF-κB were simultaneously deactivated by kaempferol in acute inflammation, as shown by carrageenan-induced mouse paw edema model. The concomitant reduction in COX-2 expression in paw tissues suggested kaempferol's role in mitigation of inflammation by targeting STAT3 and NF-κB.
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