Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5507015 | Biochemistry and Biophysics Reports | 2017 | 8 Pages |
â¢IgE producing hybridomas aggregated in the presence of antigen and secreted IgE.â¢FcγRs but not FcεRs were required for the cell aggregation.â¢The cell aggregation with LPS stimulation enhanced IgE production.â¢IgE producing plasma cells formed the cell aggregates in spleen of immunized mice.â¢Inhibition of FcγRs reduced the IgE-producing plasma cell aggregation and serum IgE.
Allergic conditions result in the increase of immunoglobulin (Ig)E-producing plasma cells (IgE-PCs); however, it is unclear how IgE production is qualitatively controlled. In this study, we found that IgE-PCs in spleen of immunized mice formed homotypic cell aggregates. By employing IgE-producing hybridomas (IgE-hybridomas) as a model of IgE-PCs, we showed that these cells formed aggregates in the presence of specific antigens (Ags). The formation of the Ag-induced cell aggregation involved secreted IgE and Fcγ receptor (FcγR)II/FcγRIII, but not FcεRs. Ag-induced cell aggregation plus lipopolysaccharide signaling resulted in an enhancement of IgE production in aggregated IgE-hybridomas. Furthermore, the administration of anti-FcγRII/FcγRIII antagonistic monoclonal antibody to immunized mice tended to reduce the splenic IgE-PC aggregation as well as the serum IgE levels. Taken together, our results suggested that Ag-IgE complexes induced IgE-PCs aggregation via FcγRII/FcγRIII, leading to the enhancement of IgE production. These findings suggest the presence of a novel mechanism for regulation of IgE production.