Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5507039 | Biochemistry and Biophysics Reports | 2017 | 6 Pages |
Abstract
Diverse biological events are regulated through protein phosphorylation mediated by protein kinases. Some of these protein kinases are known to be involved in the pathogenesis of various diseases. Although 518 protein kinase genes were identified in the human genome, it remains unclear how many and what kind of protein kinases are expressed and activated in cells and tissues under varying situations. To investigate cellular signaling by protein kinases, we developed monoclonal antibodies, designated as Multi-PK antibodies, that can recognize multiple protein kinases in various biological species. These Multi-PK antibodies can be used to profile the kinases expressed in cells and tissues, identify the kinases of special interest, and analyze protein kinase expression and phosphorylation state. Here we introduce some applications of Multi-PK antibodies to identify and characterize the protein kinases involved in epigenetics, glucotoxicity in type 2 diabetes, and pathogenesis of ulcerative colitis. In this review, we focus on the recently developed technologies for kinomics studies using the powerful analytical tools of Multi-PK antibodies.
Keywords
2D-PAGEFAKDNA methyltransferase 1IEFKinomicsCaMKDclkMecp2CDKL5CNBrDnmt1cyclin-dependent kinase-like 5IPGimmobilized pH gradientMAPKMonoclonal antibodytwo-dimensional polyacrylamide gel electrophoresiscyanogen bromideisoelectric focusingProtein phosphorylationProteomicsProtein kinasemitogen-activated protein kinaseCa2+/calmodulin-dependent protein kinasefocal adhesion kinase
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Authors
Yasunori Sugiyama, Isamu Kameshita,