Dynamic approach to predict pH profiles of biologically relevant buffers

•Accurate model proposed for Phosphate and Universal buffer using dynamic approach.•Optimization of pKa values in phosphate and universal buffer using dynamic approach.•A method that than be easily extended to predict pH of complex bio-fluids if concentration of metabolites and its pKa values are known.

Recently, dynamic approach has been applied to determine the steady state concentrations of multiple ionic species present in complex buffers at equilibrium. Here, we have used the dynamic approach to explicitly model the pH profiles of biologically relevant phosphate buffer and universal buffer (a mixture of three tri-protic acids such as citric acid, boric acid and phosphoric acid). The results from dynamic approach are identical to that of the conventional algebraic approach, but with an added advantage that the dynamic approach, allow for the modelling of complex buffer systems relatively easy compared to that of algebraic method.

Graphical abstractDownload high-res image (97KB)Download full-size image