| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5507099 | Biochemistry and Biophysics Reports | 2017 | 6 Pages |
â¢Titin and myomesin as well as their domains stimulate myoblast proliferation.â¢Ig-like and Fn type III domains activate proliferation via different pathways.â¢IGF-1 autocrine signalling is partially involved in observed proliferative effects.
Myofibrillar proteins titin and myomesin stimulated myoblast proliferation as determined by MTT-test and labelled thymidine incorporation in the DNA. Specific Fn type III and Ig-like domains of these proteins were able to exert mitogenic effects as well. Proliferative effect of Fn type III domains was highly sensitive to inhibition of Ca2+/calmodulin dependent protein kinase, whereas the effect of Ig-like domains showed greater sensitivity to the inhibition of adenylyl cyclase - cAMP - PKA pathway. IGF-1 autocrine signalling inhibition partially suppressed mitogenic effects revealed by both domain types.
