| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5507951 | Biochimica et Biophysica Acta (BBA) - General Subjects | 2017 | 13 Pages |
Abstract
Fibroblasts in tensioned collagen gels altered their phenotypes in a MF rate-dependent manner. Collagen gel culture with tension and MF could be utilized as an appropriate in vitro model of interstitial connective tissues to evaluate the pathophysiological significance of mechanosignals generated by fluid flow and cellular/extracellular tension.
Keywords
ECMqRT-PCRTissue tensionPFA2-DDABDMEMFBSPCNA3,3′-diaminobenzidineCGCH&EDulbecco's modified Eagle's mediumdistilled waterAdenosine TriphosphateATPProliferating Cell Nuclear AntigenTensile strengthMasson's trichromeEnzyme-linked immunosorbent assayELISAInterstitial fluid flowfetal bovine serumStatic culturecollagen gel cultureExtracellular matrixinterstitial fluidHematoxylin and EosinHyaluronanquantitative real-time reverse transcription polymerase chain reaction
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Authors
Natsumi Saito, Hiroaki Adachi, Hiroshi Tanaka, Satoru Nakata, Norifumi Kawada, Ken Oofusa, Katsutoshi Yoshizato,