Effect of therapeutic concentration of lithium on live HEK293 cells; increase of Na+/K+-ATPase, change of overall protein composition and alteration of surface layer of plasma membrane

Article ID	Journal	Published Year	Pages	File Type
5508111	Biochimica et Biophysica Acta (BBA) - General Subjects	2017	32 Pages	PDF

Abstract

Our findings demonstrate adaptation of live HEK293 cell metabolism to prolonged exposure to therapeutic concentration of Li manifested as up-regulation of Na+/K+-ATPase expression, alteration of protein composition and change of the surface layer of plasma membrane.

Keywords

N-methyl-d-aspartate TFA NCS TOF CBB dMPC Laurdan ABC ECL HEK293 GSK-3β NMDA PMSF PoPC DPH SLB DMPS DPPS TMA-DPH λex 6-dodecanoyl-2-dimethylaminonaphthalene DLPs dilauroylphosphatidylserine post nuclear supernatant SDS PBS TCSPC ACN Lithium 1,6-diphenyl-1,3,5-hexatriene Electrogenerated chemiluminescence FLIM MS/MS Na+/K+-ATPase Sodium-potassium adenosine triphosphatase POPs bipolar disorder Acetonitrile Trifluoroacetic acid 2D electrophoresis Two-dimensional electrophoresis SDS-PAGE sodium dodecyl sulphate polyacrylamide gel electrophoresis large unilamellar vesicles Ammonium bicarbonate Membrane biophysics Proteomic analysis Trypsinization matrix-assisted laser desorption/ionization dipalmitoylphosphatidylserine Molecular dynamics Relative humidity Time-of-Flight sodium dodecyl sulphate newborn calf serum HEK293 cells human embryonic kidney 293 cells endoplasmic reticulum time-correlated single-photon counting Fluorescence lifetime Excitation wavelength Mass spectrometry Tandem mass spectrometry Phosphate buffered saline phosphatidylinositol phenylmethanesulphonyl fluoride LUV MALDI hexokinase Geneticin PNS Creatine kinase liquid chromatography high performance liquid chromatography HPLC Glycogen synthase kinase-3β