Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5508518 | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids | 2017 | 41 Pages |
Abstract
In the yeast Saccharomyces cerevisiae, the mitochondrial phosphatidylserine decarboxylase 1 (Psd1p) produces the largest amount of cellular phosphatidylethanolamine (PE). Psd1p is synthesized as a larger precursor on cytosolic ribosomes and then imported into mitochondria in a three-step processing event leading to the formation of an α-subunit and a β-subunit. The α-subunit harbors a highly conserved motif, which was proposed to be involved in phosphatidylserine (PS) binding. Here, we present a molecular analysis of this consensus motif for the function of Psd1p by using Psd1p variants bearing either deletions or point mutations in this region. Our data show that mutations in this motif affect processing and stability of Psd1p, and consequently the enzyme's activity. Thus, we conclude that this consensus motif is essential for structural integrity and processing of Psd1p.
Keywords
MPPDMPEToMSRSphosphatidic acidtranslocase of the outer mitochondrial membranedimethylphosphatidylethanolamineSaccharomyces cerevisiaesubstrate recognition siteendoplasmic reticulumphosphatidylinositolphosphatidylcholinephosphatidylethanolaminePhosphatidylserinePhospholipidLysophospholipidsMitochondriamitochondrial processing peptidaseCardiolipin
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Authors
Francesca Di Bartolomeo, Kim Nguyen Doan, Karin Athenstaedt, Thomas Becker, Günther Daum,