Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5508654 | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research | 2017 | 23 Pages |
Abstract
GFP-Aequorin Protein (GAP) can be used to measure [Ca2Â +] inside intracellular organelles, both by luminescence and by fluorescence. The low-affinity variant GAP3 is adequate for ratiometric imaging in the endoplasmic reticulum and Golgi apparatus, and it can be combined with conventional synthetic indicators for simultaneous measurements of cytosolic Ca2Â +. GAP is bioorthogonal as it does not have mammalian homologues, and it is robust and functionally expressed in transgenic flies and mice, where it can be used for Ca2Â + measurements ex vivo and in vivo to explore animal models of health and disease. This article is part of a Special Issue entitled: ECS Meeting edited by Claus Heizmann, Joachim Krebs and Jacques Haiech.
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Authors
MarÃa Teresa Alonso, Jonathan Rojo-Ruiz, Paloma Navas-Navarro, Macarena RodrÃguez-Prados, Javier GarcÃa-Sancho,