Comparability of the effect of storage time and temperature on serum anti-Müllerian hormone measurement between original and modified enzyme-linked immunosorbent assay

Pre-mixing protocol did not influence the results of fresh serum or frozen serum incubation < 3 days at 4 °C and − 80 °C, but when specimens detected after collection and stored in other storage conditions, should be pre-mixed with assay buffer to insure its accuracy.