Performance of a phosphoflow assay to determine phosphorylation of S6 ribosomal protein as a pharmacodynamic read out for mTOR inhibition

Article ID	Journal	Published Year	Pages	File Type
5510083	Clinical Biochemistry	2016	27 Pages	PDF

Abstract

The phosphoflow assay of p-S6RP performed well analytically, but sample stability, specificity, and method comparison results question its fitness for clinical purposes.

Keywords

PBMCs ERK mTOR KTx MPA mTORi RSK p70S6K1 PBS phorbol 12-myristate 13-acetate PI3K EVR BSA LC-MS/MS PMA MAPK bovine serum albumin Akt EDTA Ethylenediaminetetraacetic acid Everolimus CSA ELISA Kidney transplant recipients peripheral blood mononuclear cells Cyclosporine A Coefficient of Variation Liquid chromatography-tandem mass spectrometry phosphoinositide 3-kinase mycophenolic acid Phosphate-buffered saline mTOR inhibitors mammalian target of rapamycin C-reactive protein CRP protein kinase B mitogen-activated protein kinase extracellular signal-regulated kinase

Related Topics

Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry

Performance of a phosphoflow assay to determine phosphorylation of S6 ribosomal protein as a pharmacodynamic read out for mTOR inhibition

Authors

Emaad Abdel-Kahaar, Mariana Kabakchiev, Bertram Hartmann, Eberhard Wieland, Maria Shipkova,