Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5511907 | International Journal of Biological Macromolecules | 2017 | 24 Pages |
Abstract
Low molecular weight fractions were derived from native high molecular weight dextran produced by Leuconostoc mesenteroides KIBGE-IB26. Structural characterization of native and low molecular weight fractions obtained after acidic and enzymatic hydrolysis was done using FTIR and NMR spectroscopy. The molecular weight was estimated using Diffusion Ordered NMR spectroscopy. Native dextran (892 kDa) is composed of α-(1 â 6) glycosidic linkage along with α-(1 â 3) branching. Major proportion of 528 kDa dextran was obtained after prolong enzymatic hydrolysis however, an effective acidic treatment at pH-1.4 up to 02 and 04 h of exposure resulted in the formation of 77 kDa and 57 kDa, respectively. The increment in pH from 1.4 to 1.8 lowered the hydrolysis efficiency and resulted in the formation of 270 kDa dextran fraction. The results suggest that derived low molecular weight water soluble fractions can be utilized as a drug delivery carrier along with multiple application relating pharmaceutical industries.
Keywords
EPSTOCSYHSQC2D NMRExopolysaccharideNMRFTIRdiffusion-ordered spectroscopyNuclear magnetic resonance spectroscopyTotal correlation spectroscopyheteronuclear single-quantum correlation spectroscopyheteronuclear multiple-bond correlation spectroscopyHomonuclear correlation spectroscopyDOSYFourier-transform infraredSEMStructural characterizationScanning electron microscopyHMBC یا Heteronuclear Multiple Bond CorrelationEnzymatic hydrolysisAcid hydrolysisCOSY
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Authors
Samina Iqbal, Roberta Marchetti, Afsheen Aman, Alba Silipo, Shah Ali Ul Qader, Antonio Molinaro,