| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5512106 | International Journal of Biological Macromolecules | 2017 | 28 Pages |
Abstract
Chitosan was extracted from the pen of squid Doryteuthis singhalensis and characterized using FT-IR, NMR, CHN, SEM and DSC analysis. Purified chitosan was sulfated with chlorosulfonic acid in N,N-dimethylformamide and the added sulfate group was confirmed with FT-IR analysis. The molecular weight and degree of deacetylation (DDA) of chitosan was found 226.6Â kDa and 83.76% respectively. Chitosan exhibited potent antioxidant activity evidenced by reducing power, chelating ability on ferrous ions and scavenging activity on DPPH, superoxide and hydroxyl radicals. The anticoagulant assay using activated partial thromboplastin time (APTT) and prothrombin time (PT) showed chitosan as a strong anticoagulant. The results of this study showed possibility of using D. singhalensis pen as a non-conventional source of natural antioxidants and anticoagulant which can be incorporated in functional food formulations.
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Authors
Pasiyappazham Ramasamy, Namasivayam Subhapradha, Thangadurai Thinesh, Joseph Selvin, Kanagaraj Muthamizh Selvan, Vairamani Shanmugam, Annaian Shanmugam,