Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5512407 | International Journal of Biological Macromolecules | 2017 | 19 Pages |
Abstract
Injectable hydrogels have a variety of applications, including regenerative medicine, tissue engineering and controlled drug delivery. In this paper, we reported on a pure protein hydrogel based on tetrameric recombinant proteins for the potential drug delivery application. This protein hydrogel was formed instantly by simply mixing two recombinant proteins (ULD-TIP1 and ULD-GGGWRESAI) through the specific protein-peptide interaction. The protein hydrogel was characterized by rheology and scanning electron microscopy (SEM). In vitro cytotoxicity test indicated that the developed protein hydrogel had no apparent cytotoxicity against L-929 cells and HCEC cells after 48Â h incubation. The formed protein hydrogels was gradually degraded after incubation in phosphate buffered solution (PBS, pHÂ =Â 7.4) for a period of 144Â h study, as indicated by in vitro degradation test. Encapsulation of model drug (sodium diclofenac; DIC) were achieved by simple mixing of drugs with hydrogelator and the entrapped drugs was almost completely released from hydrogels within 24Â h via a diffusion manner. As a conclusion, the simple and mild preparation procedure and good biocompatibility of protein hydrogel would render its good promising candidate for drug delivery applications.
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Authors
Xu Xu, ZhaoKang Xu, XiaoFeng Yang, YanHao He, Rong Lin,