Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5512478 | International Journal of Biological Macromolecules | 2017 | 11 Pages |
Abstract
In this study, the interaction of three [1,2,3,4]tetrazolo[5,1-b]-quinazolin-8-one derivatives with salmon sperm DNA and BSA was investigated experimentally and theoretically. Fluorescence and absorption spectroscopy techniques were applied to determine the probable interaction mechanism and correlated binding constants and thermodynamic parameters. It was found that the compounds intercalate into the DNA duplex via minor groove in a moderately strong fashion with the binding constants of 104Â Mâ1. The values of binding constant for the interaction with BSA at different temperatures were also calculated to be in the range of 1.06Â ÃÂ 103-3.54Â ÃÂ 108Â Mâ1 indicating the relatively high propensity of the compounds to BSA. In vitro cytotoxicity studies on the effect of the compounds on MCF-7 and HT-29 cancerous cell lines as well as DPSC normal cell line were performed using MTT assay. It was observed that the compounds exhibited selective inhibitory effect against cancer cell growth. Moreover, each of the title compounds was separately docked to DNA and BSA using Autodock Vina as a molecular docking program. The obtained theoretical results were in good agreement with those obtained from Experimental studies.
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Authors
Maryam Mohamadi, Asadollah Hassankhani, S. Yousef Ebrahimipour, Masoud Torkzadeh-Mahani,