Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5513413 | Methods | 2017 | 9 Pages |
Abstract
Here we describe the synthesis and use of a directed hydroxyl radical probe, tethered to a pre-mRNA substrate, to map the structure of this substrate during the spliceosome assembly process. These studies indicate an early organization and proximation of conserved pre-mRNA sequences during spliceosome assembly. This methodology may be adapted to the synthesis of a wide variety of modified RNAs for use as probes of RNA structure and RNA-protein interaction.
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Authors
Charnpal S. Grewal, Oliver A. Kent, Andrew M. MacMillan,