| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5513418 | Methods | 2017 | 7 Pages |
â¢Crystal structure analysis of spliceosomal helicases.â¢Structural information obtained by Cryo-EM.â¢Methods to measure ATPase and helicase activities in vitro.
The splicing of eukaryotic precursor mRNAs requires the activity of at least three DEAD-box helicases, one Ski2-like helicase and four DEAH-box helicases. High resolution structures for five of these spliceosomal helicases were obtained by means of X-ray crystallography. Additional low resolution structural information could be derived from single particle cryo electron microscopy and small angle X-ray scattering. The functional characterization includes biochemical methods to measure the ATPase and helicase activities. This review gives an overview on the techniques used to gain insights in to the structure and function of spliceosomal helicases.
