Recent adaptations of fluorescence techniques for the determination of mechanistic parameters of helicases and translocases

Article ID	Journal	Published Year	Pages	File Type
5513615	Methods	2016	16 Pages	PDF

Keywords

N-acetyl-L-tryptophanamide NATA PEP Helicase BLM TIRF NTP MDCC Translocase Trp DNA ATP RNA Nucleic Acid Translocation Förster resonance energy transfer FRET Unwinding WHD Thermodynamics Tryptophan single-stranded base pair winged helix domain double-stranded Dextran sulfate Kinetics inorganic phosphate phosphoenolpyruvate lactate dehydrogenase LDH MANT Mechanism NADH Nucleotide nucleoside triphosphate NAD, nicotinamide adenine dinucleotide Hydrolysis pyruvate kinase total internal reflection fluorescence

Related Topics

Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry

Recent adaptations of fluorescence techniques for the determination of mechanistic parameters of helicases and translocases

Authors

Máté Gyimesi, Gábor M. Harami, Zsuzsa S. Kocsis, Mihály Kovács,