Article ID Journal Published Year Pages File Type
5516072 Protein Expression and Purification 2017 9 Pages PDF
Abstract

•Expression of untagged wild-type and mutant human superoxide dismutase 1 (SOD1) proteins in the periplasm of E. coli.•Simple and cheap purification of human SOD1 proteins from the periplasm of E. coli.•Characterization of purified SOD1 proteins by SDS-PAGE and enzyme activity.•Characterization of SOD1 aggregation by Thioflavin T binding, SDS-PAGE, electron microscopy, and cellular toxicity.

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Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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An easy method for bacterial expression and purification of wild-type and mutant superoxide dismutase 1 (SOD1)
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